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KMID : 1024420110150030276
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Food Engineering Progress
2011 Volume.15 No. 3 p.276 ~ p.281
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Optimization of Extraction and Purification of Phytic Acid from Defatted Rice Bran
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Choi Moon-Sil
Han Bok-Kyung
Choi Hyuk-Joon
Park Young-Seo
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Abstract
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The optimum condition for the extraction and purification processes of phytic acid from defatted rice bran was examined. The phytic acid was efficiently extracted when the defatted rice bran was treated with 10 volumes of 0.5% HCl for 1 hr. For the neutralization of acid-treated extract, 0.5% NaOH was the most acceptable. To purify phytic acid, Diaion HP20 resin was used to remove impurities from the extract. The flow-through was then loaded onto ion exchange columns packed with various resins and among them, Amberlite IRA-416 resin showed highest recovery yield. When the phytic acid was absorbed onto Amberlite IRA-416 resin and then eluted with 0.5% NaOH, 89% of applied phytic acid was eluted. Most proteins were removed from the purified phytic acid and total protein content of the phytic acid was 0.14% (w/w).
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KEYWORD
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phytic acid, defatted rice bran, extraction, ion exchange column chromatography
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